电离辐射通过转化生长因子-β-介导的内膜-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简要 ：探讨宇宙射直通有否可通过转化酪氨酸-β（TGF-β）-内皮细胞的结缔四组织-糖蛋白转换 (EMT)来促进恶性肿瘤的波及移至。应用于分之一2Gy(60)Coγ直通反射源自人类所器官的6种恶性肿瘤，记录与EMT相关的变化，这之外分别利用光学仪器高效率，核酸印迹新方例，免疫荧光高效率，划痕检验和Transwell四人检验来观察并扫描细胞四组织形态，EMT标上，波及移至控制能力等。引入核糖体亦同免疫吸附例扫描这些恶性肿瘤之中TGF-β蛋白水平，利用特别酶抑制剂SB431542来风险评估TGF-β瞬时路中在宇宙射直通EMT之中的作用。经过分之一为2Gy反射的恶性肿瘤之中存在间叶细胞的表达，与；也反射四组相比其结缔四组织标上减少，间叶细胞标上增加，同时其波及移到控制能力增强，TGF-β蛋白水平也提高。进一步推测由A549宇宙射直通诱导的EMT可通过对TGF-β瞬时抑制暴发再一。这些结果表明TGF-β内皮细胞的EMT在宇宙射直通诱导增强恶性肿瘤波及移到控制能力之中起着不可忽视。

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